The Soluble Cytochrome Oxidase of Nitrosomonas europaea
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چکیده
منابع مشابه
Cytochrome aa3 from Nitrosomonas europaea.
Cytochrome c oxidase has been purified from the ammonia oxidizing chemoautotroph Nitrosomonas europaea by ion-exchange chromatography in the presence of Triton X-100. The enzyme has absorption maxima at 420 and 592 nm in the resting state and at 444 and 598 nm in the dithionite-reduced form; optical extinction coefficient (598 nm minus 640 nm) = 21.9 cm-1 nM-1. The enzyme has approximately 11 n...
متن کاملMethane oxidation by Nitrosomonas europaea.
Methane inhibited NH4+ utilization by Nitrosomonas europaea with a Ki of 2mM. O2 consumption was not inhibited. In the absence of NH4+, or with hydrazine as reductant, methane caused nearly a doubling in the rate of O2 uptake. The stimulation was abolished by allylthiourea, a sensitive inhibitor of the oxidation of NH4+. Analysis revealed that methanol was being formed in these experiments, wit...
متن کاملStructure of the Nitrosomonas europaea Rh protein.
Amt/MEP/Rh proteins are a family of integral membrane proteins implicated in the transport of NH3, CH(2)NH2, and CO2. Whereas Amt/MEP proteins are agreed to transport ammonia (NH3/NH4+), the primary substrate for Rh proteins has been controversial. Initial studies suggested that Rh proteins also transport ammonia, but more recent evidence suggests that they transport CO2. Here we report the fir...
متن کاملNO reductase activity of the tetraheme cytochrome C554 of Nitrosomonas europaea.
The tetraheme cytochrome c(554) (cyt c(554)) from Nitrosomonas europaea is believed to function as an electron-transfer protein from hydroxylamine oxidoreductase (HAO). We show here that cyt c(554) also has significant NO reductase activity. The protein contains one high-spin and three low-spin c-type hemes. HAO catalyzed reduction of the cyt c(554), ligand binding, intermolecular electron tran...
متن کاملPhosphorylation by extracts of Nitrosomonas europaea.
Burge, W. D. (University of California, Berkeley), E. Malavolta, and C. C. Delwiche. Phosphorylation by extracts of Nitrosomonas europaea. J. Bacteriol. 85:106-110. 1963.-Cellfree preparations of Nitrosomonas europaea are capable of oxidizing hydroxylamine, but not ammonium ion, to nitrite. The quantity of nitrite formed by our preparations was, at most, equivalent to only 70% of the hydroxylam...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1983
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-129-6-1645